DICAST Outputs

DICAST provides outputs as you would expect them from each Alternative Splicing tool within output/<astoolname>-output/<Fastq-filename>_output.

DICAST also provides a output/<astoolname>-output/<Fastq-filename>_output_dicast_unified output format for each tool. This is a simple tsv file that hosts all the events found from each tool. We used this to unify the outputs needed to build each of the plots outputted by DICAST.

output/
├── <astoolname>-output
│   ├── logs
│   ├── <Fastq-filename>_output
│   ├── <Fastq-filename>_output_<astoolname>_dicast_unified
└── plots
    └── <Fastq-filename>
        ├── <mapping tool>-name
        │   ├── A3_compare.png
        │   ├── A5_compare.png
        │   ├── AFE_compare.png
        │   ├── ALE_compare.png
        │   ├── ES_compare.png
        │   ├── IR_compare.png
        │   ├── MEE_compare.png
        │   ├── MES_compare.png
        │   └── overall_compare.png
        └── unmapped
            ├── A3_compare.png
            ├── A5_compare.png
            ├── AFE_compare.png
            ├── ALE_compare.png
            ├── ES_compare.png
            ├── IR_compare.png
            ├── MEE_compare.png
            ├── MES_compare.png
            └── overall_compare.png

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DICAST also outputs an UpSet plot for each Fastq-filename-mapping_tool combination.

This plot shows the events that were found in common by tools and shows you which tools found these events as well.

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When run with ASimulatoR, DICAST also outputs precision and recall plots for each Fastq-filename-mapping_tool combination.;

for all events

and for each event